Post-Doc : Deciphering the crosstalk between human macrophages and Tfh cells in steadystate and in B cell lymphomas

Post-Doc : Deciphering the crosstalk between human macrophages and Tfh cells in steadystate and in B cell lymphomas (France, Brittany, Rennes)

A 2 years post-doctoral position is available at the INSERM UMR1236 headed by the Pr Karin TARTE (Rennes, France, https://micmac.univ-rennes1.fr/en) to work within the frame
of B-cell lymphoma physiopathology.
The research project of the unit is divided in two axes: i) study of normal B cell differentiation and deregulation of these mechanisms during lymphomagenesis, ii) study of lymph node microenvironment and in particular lymphoid stromal cells, myeloid cells, and follicular helper T-cells and their deregulation during lymphomagenesis. Successful applicant will join our multidisciplinary team located within the Health Campus in Rennes (https://www.tourisme-rennes.com/en). In this environment he/she will access to state-ofthe-art facilities (10X enomics & C1_Biomark, Flow cytometry/Cell-sorting/CYTOF, microscopy platform…) for conducting this research project and to our unique lymphoma tissue bank including frozen viable samples suitable for functional analysis.

The proposed project aims to characterize the crosstalk between human macrophages and Tfh cells in steady-state and in B cell lymphomas. T follicular helper (Tfh) cells are essential for efficient humoral responses. We hypothesize that macrophages (Mac) play a major role in the induction of Tfh cells in human lymphoid organs and that an altered Mac/Tfh dialogue is involved in the pathogenesis of FL, a disease strongly dependent on a specific tumor-supportive niche. We plan to combine unbiased methods for single-cell analysis (scRNA-seq, CyTOF, high dimensional imaging [Hyperion]), functional assays using cells directly purified from human samples (nonpathological and B cell lymphomas) and relevant in vitro culture models. This project will provide novel insight into the role of macrophages in the induction of normal humoral responses in human.

The applicant should be a highly motivated scientist with a solid background in immunology. Skills in large-scale RNAseq approaches, flow cytometry/CyTOF, and tissue imaging would be appreciated but each of them is not strictly required. The project will involve close cooperation with other biologists and bioinformaticians. The lab is operating in French and English. The position should start early 2020.

Interested applicants should submit a CV and list of publications, a statement of research interests, and the names of two referees with contact information to Dr Mikael Roussel
(mikael.roussel@chu-rennes.fr) and Pr Karin Tarte (karin.tarte@univ-rennes1.fr).